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A Doody's Core Title for 2015.
Molecular Biology, 5/e by Robert Weaver, is designed for an introductory course in molecular biology. Molecular Biology 5/e focuses on the fundamental concepts of molecular biology emphasizing experimentation. In particular author, Rob Weaver, focuses on the study of genes and their activities at the molecular level. Through the combination of excellent illustrations and clear, succinct writing students are presented fundamental molecular biology concepts.
This page intentionally left blank This page intentionally left blank wea25324_fm_i-xx.indd Page i 12/22/10 10:16 PM user-f468 /Volume/208/MHCE016/san74946_disk1of1/0073374946/san74946_pagefiles Molecular Biology Fifth Edition R o b e r t F. We a v e r University of Kansas TM wea25324_fm_i-xx.indd Page ii 30/12/10 5:25 PM user-f467 /Volume/208/MHCE016/san74946_disk1of1/0073374946/san75292_pagefile TM MOLECULAR BIOLOGY, FIFTH EDITION Published by McGraw-Hill, a business unit of The
and colleagues used smaller fragments of the 1–550 region to pinpoint the part of b9 that was inducing the binding. All of the fragments illustrated in Figure 6.24 could induce binding, although the 260–550 fragment would work only at low temperature. Strikingly, the very small 262–309 fragment, with only 48 amino acids, could stimulate binding very actively, even at room temperature. Mutations in three amino acids in this region (R275, E295, and A302) were already known to interfere with s
emissions and analyzes them electronically, so the difference between 10,000 dpm and 50,000 dpm would be obvious. Here is how this technique works: One starts with a radioactive sample—a blot with RNA bands that have hybridized with a labeled probe, for example. This sample is placed in contact with a phosphorimager plate, which absorbs b electrons. These electrons excite molecules on the plate, and these molecules remain in an excited state until the phosphorimager scans the plate with a laser.
these fragments (labeled A, B, and C according to size) contain the minisatellites, represented by blue boxes. The other fragments (yellow) contain unrelated DNA sequences. (b) Electrophorese the fragments from part (a), which separates them according to their sizes. All eight fragments are present in the electrophoresis gel, but they remain invisible. The positions of all the fragments, including the three (A, B, and C) with minisatellites are indicated by dotted lines. (c) Denature the DNA
drawback of the pyrosequencing technique is that each read on a given piece of DNA can currently go only about 200–300 nt before the sequence accuracy is unacceptably degraded. In the liquid version of the procedure, this degradation comes from dilution of the sample by repeated additions of reagents, and buildup of inhibitory products, as well as the fact that some chains inevitably get ahead of the majority, and some fall behind. With increasing chain length, these asynchronous chain